Journal: PLoS ONE

Article Title: Role of Constitutive Behavior and Tumor-Host Mechanical Interactions in the State of Stress and Growth of Solid Tumors

doi: 10.1371/journal.pone.0104717

Figure Lengend Snippet: Experimentally measured elastic stress-strain response of MCF10CA1a and SW620 tumors in unconfined compression. Data show individual tumor behavior.

Article Snippet: Two cancer cell lines were used in this study: the human breast cancer cell line MCF10CA1a, derived from in vivo passaging of H-Ras transformed MCF10A cells and the metastatic human colon adenocarcinoma cell line SW620 (ATCC).

Techniques:

Journal: PLoS ONE

Article Title: Role of Constitutive Behavior and Tumor-Host Mechanical Interactions in the State of Stress and Growth of Solid Tumors

doi: 10.1371/journal.pone.0104717

Figure Lengend Snippet: Experimental measurements for the elastic modulus and tan( δ ) of the two tumor types.

Article Snippet: Two cancer cell lines were used in this study: the human breast cancer cell line MCF10CA1a, derived from in vivo passaging of H-Ras transformed MCF10A cells and the metastatic human colon adenocarcinoma cell line SW620 (ATCC).

Techniques:

Journal: PLoS ONE

Article Title: Role of Constitutive Behavior and Tumor-Host Mechanical Interactions in the State of Stress and Growth of Solid Tumors

doi: 10.1371/journal.pone.0104717

Figure Lengend Snippet: Values of the mechanical properties of the two tumor types derived by fitting the model to the experimental stress-strain curves.

Article Snippet: Two cancer cell lines were used in this study: the human breast cancer cell line MCF10CA1a, derived from in vivo passaging of H-Ras transformed MCF10A cells and the metastatic human colon adenocarcinoma cell line SW620 (ATCC).

Techniques: Derivative Assay

Journal: PLoS ONE

Article Title: Role of Constitutive Behavior and Tumor-Host Mechanical Interactions in the State of Stress and Growth of Solid Tumors

doi: 10.1371/journal.pone.0104717

Figure Lengend Snippet: A) Model fit to the experimentally measured growth curve of SW620 tumors using the neo-Hookean and the exponential equation. B) Evolution of bulk solid stress in the tumor interior does not depend on the selection of the constitutive equation. Results using the Blatz-Ko material are omitted for clarity.

Article Snippet: Two cancer cell lines were used in this study: the human breast cancer cell line MCF10CA1a, derived from in vivo passaging of H-Ras transformed MCF10A cells and the metastatic human colon adenocarcinoma cell line SW620 (ATCC).

Techniques: Selection

Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: Cytotoxic effect of 5-FU/Curcumin hybrids on SW620, HaCaT, and CHO-K1 cell lines.

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques:

Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: Data of viability (%) at the lowest and the highest concentrations evaluated.

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques: Concentration Assay

Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: ( A ). Antiproliferative activity of hybrids 6a and 6d in SW480 cells; ( B ). Antiproliferative activity of hybrids 6d and 6e in SW620 cells. Data are presented as the mean ± SE of three independent experiments. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). Vehicle control was assumed to be 100% viable.

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques: Activity Assay, Control

Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: Cell cycle distribution after treatment with hybrids 6d and 6e in SW620 cells and representation of distribution percentages in bar chart form. All experiments were performed in duplicate and showed similar results. p -values lower than 0.05 were considered statistically significant (** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques:

Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: Changes in ΔΨm and plasma membrane integrity. Treatment with hybrids 6a and 6d in SW480 and hybrids 6d and 6e in SW620 cells. PI+: PI-positive cells, with membrane damage or dead cells; DiOC6 High: live cells with high membrane polarization; DiOC6 Low: cells in latency that lose membrane polarization. Data are presented as the mean of these two populations ± SE of three independent experiments. p values lower than 0.05 were considered statistically significant (** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques: Clinical Proteomics, Membrane

Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: Apoptosis of SW480 ( A ) and SW620 ( B ) cells following treatment with the hybrid compounds 6a , 6d , and 6e . The histograms represent one of at least two different experiments. Q1 + Q2: Cells that lost membrane integrity (late apoptotic cells, dead cells, necroptotic cells, secondary necrotic cells, others); Q3: Early apoptotic cells; Q4: Viable cells. Data are presented as the mean of these two populations ± SE of three independent experiments. p -values lower than 0.05 were considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques: Membrane

Journal: Pharmaceutics

Article Title: New Hybrid Scaffolds Based on 5-FU/Curcumin: Synthesis, Cytotoxic, Antiproliferative and Pro-Apoptotic Effect

doi: 10.3390/pharmaceutics15041221

Figure Lengend Snippet: Effect of 6d and 6e hybrids on apoptotic biomarker expression in SW620 cells. Results are the mean ± SE of two independent experiments. p -values less than 0.05 were considered statistically significant (* p < 0.05; ** p < 0.01).

Article Snippet: SW480 (colon adenocarcinoma) and SW620 (derived metastatic) malignant cell lines, and the non-malignant lines HaCaT (human keratinocytes) and CHO-K1 (Chinese Hamster Ovary), were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Porton Down, Salisbury, UK).

Techniques: Biomarker Discovery, Expressing

Journal: Oncology Letters

Article Title: A gut butyrate-producing bacterium Butyricicoccus pullicaecorum regulates short-chain fatty acid transporter and receptor to reduce the progression of 1,2-dimethylhydrazine-associated colorectal cancer

doi: 10.3892/ol.2020.12190

Figure Lengend Snippet: Effects of butyrate on CRC cells. (A) Effect of conditioned medium of B. pullicaecorum cultivation on CRC cell proliferation. Numbers of SW480 and SW620 cells were counted at days 1 and 5 following cell seeding. One-way ANOVA with Fisher's least significant difference (LSD) post-hoc test was performed to identify significant differences between groups. (B) Relative mRNA levels of SLC5A8. (C) Relative mRNA levels of GPR43. Gene quantitation was performed from SW480 cells and SW620 cells treated with 5 mM NaB for 48 h. Differences in gene expressions were compared using Student's t-test. Data are presented as mean ± SEM. *P<0.05. **P<0.01. CRC, colorectal cancer; NaB, sodium butyrate; SLC5A8, solute carrier family 5 member 8; GPR43, G-protein-coupled receptor 43; PY-X, peptone yeast extract broth; HRC, human reference cDNA.

Article Snippet: SW480 colon cancer cell line (ATCC CRL-1459; AJCC stage II) and SW620 lymph node metastatic derivative cell line (ATCC CRL-1831; AJCC stage III) from the same patient were purchased from American Type Culture Collection.

Techniques: Quantitation Assay

Journal: iScience

Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions

doi: 10.1016/j.isci.2025.114150

Figure Lengend Snippet: Validation of Knockdown Efficiency of ATP10A and DBI (A and B) Western blot analysis confirming the knockdown efficiency of ATP10A and DBI in the SW620 and DLD-1 cell lines. (C–F) qRT-PCR validation of ATP10A and DBI knockdown efficiency in the SW620 and DLD-1 cell lines. Data are presented as the mean ± SD. Comparisons among four groups were analyzed by one-way ANOVA followed by Tukey post hoc test (C–F). Significance levels were denoted as follows: p < 0.001 (∗∗∗), and p < 0.0001 (∗∗∗∗).

Article Snippet: SW620 , Servicebio , Cat# STCC10812P.

Techniques: Biomarker Discovery, Knockdown, Western Blot, Quantitative RT-PCR

Journal: iScience

Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions

doi: 10.1016/j.isci.2025.114150

Figure Lengend Snippet: Effects of ATP10A and DBI Knockdown on CRC Cell Proliferation (A and B) CCK-8 assay investigating the influence of ATP10A and DBI knockdown on SW620 cell proliferation. (C and D) CCK-8 assay assessing the influence of ATP10A and DBI knockdown on DLD-1 cell proliferation. (E and F) EdU assay exploring the influence of ATP10A and DBI knockdown on SW620 cell proliferation; scale bar, 100 μm. (G and H) EdU assay evaluating the effects of ATP10A and DBI knockdown on DLD-1 cell proliferation; scale bar, 100 μm. Data are presented as the mean ± SD. Comparisons were analyzed using either two-way (A–D) or one-way (E–H) ANOVA, as appropriate, followed by Tukey post hoc test. Significance levels were denoted as follows: p < 0.05 (∗), p < 0.01 (∗∗), p < 0.001 (∗∗∗), and p < 0.0001 (∗∗∗∗); “ns” indicates no statistically significant difference.

Article Snippet: SW620 , Servicebio , Cat# STCC10812P.

Techniques: Knockdown, CCK-8 Assay, EdU Assay

Journal: iScience

Article Title: Regulatory role of phosphatidylcholine metabolic reprogramming in colorectal cancer progression and neutrophil interactions

doi: 10.1016/j.isci.2025.114150

Figure Lengend Snippet: Effects of ATP10A and DBI Knockdown on Genes Required for Neutrophil Recruitment and N2 Polarization (A–D) Changes in the expression of IL-8 and TGF-β1 following ATP10A knockdown in SW620 and DLD-1 cell lines. (E–H) Changes in the expression of IL-8 and TGF-β1 following DBI knockdown in SW620 and DLD-1 cell lines. Data are presented as the mean ± SD. Comparisons among four groups were analyzed by one-way ANOVA followed by Tukey post hoc test. Significance levels were denoted as follows: p < 0.05 (∗), p < 0.01 (∗∗), p < 0.001 (∗∗∗), and p < 0.0001 (∗∗∗∗).

Article Snippet: SW620 , Servicebio , Cat# STCC10812P.

Techniques: Knockdown, Expressing